On the Process and Review page, the Source Spectrum pane displays the source spectra specified for your Intact Mass Analysis experiment before the experiment is processed and deconvolved. For more information on selecting the source spectra, see Edit the Component Detection page and Use real-time optimization (RTO) to reprocess results.
Procedure
- Open the results of your experiment from the Queue page (for Automatic mode experiments) or from the Load Results page (for either Automatic of Manual mode experiments).
- See Open results.
- The Process and Review page opens by default and displays the results of your Intact Mass Analysis experiment, including the source spectra.
- On the Process and Review page, select the Source Spectrum tab (tabbed together with the Matched Sequence and Average DAR (if applicable) panes) on the right side of the page (if necessary).
- The Source Spectrum pane displays one source spectrum plot (for batch experiments or experiments with a single loaded raw data file) or multiple stacked plots (for multiconsensus experiments with multiple loaded raw data files).
- See Source Spectrum pane commands.
- Select the row of a component in the Results table.
- In each plot in the Source Spectrum pane, a blue line represents the m/z value of an individual charge state of the component.
- (Optional) Select the plus (+) sign to the left of a component row, and then select the row of one of the raw data files in the Results table.
- The Source Spectrum pane shows the source spectrum plot for the selected raw data file with a blue line indicating the m/z value of the top-level component.
- (Optional) Select the plus (+) sign to the left of a component (or raw data file) row, and then select the row of one of the charge states.
- The Source Spectrum pane shows the source spectrum plot for the upper-level raw data file with a blue line indicating the m/z value of the selected charge state.
- For a Sliding Windows deconvolution, the blue line represents the m/z centroid value of that individual charge state (shown the in the MZ Centroid column of the Results table).
- For an Average Over Selected Retention Time or Auto Peak Detection deconvolution, the blue line indicates the calculated monoisotopic m/z value of that charge state (shown in the Calculated Monoisotopic m/z column of the Results table) for Xtract deconvolution or the measured average m/z value of that charge state (shown in the Measured Average m/z column of the Intact Deconvolution Results table) for ReSpect deconvolution.