In the Intact Mass Analysis workflow, the BioPharma Finder application determines the molecular mass of intact (undigested) proteins and oligonucleotides. Use this workflow to deconvolve and analyze complex spectra of intact proteins and oligonucleotides using LC/MS data acquired on Thermo Scientific instruments.
The BioPharma Finder Intact Mass Analysis workflow comprises several key features, described in the table below.
Feature | Description |
|---|---|
Choose from a variety of deconvolution algorithms and methods | The BioPharma Finder application deconvolves complicated MS spectra of intact proteins or oligonucleotides using a specified algorithm (Xtract or ReSpect) and deconvolution method (Average Over Selected Retention Time, Auto Peak Detection, or Sliding Windows) to determine the molecular mass of your protein or oligonucleotide. |
Process your experiment in Automatic mode using one of the default processing methods. Alternatively, in Manual mode, first determine the optimal chromatographic peak selection and deconvolution parameters before processing your experiment. | |
Perform target sequence matching | Use the target sequence matching feature to match the measured masses of detected components to the masses of user-specified target sequences, aiding in the identification of components in your analysis. |
Generate an extracted ion chromatogram (XIC) for each component in the deconvolved spectrum to view the intensity of its signal as a function of retention time (RT). | |
Reprocess an experiment using real-time optimization (RTO) | Modify parameters and reprocess your experiment directly from the Process and Review page, where you can view updated results in real-time. |
View abundance traces for component results | For Sliding Windows experiments, you can view the abundance trace for each component in the deconvolved spectrum to quantitatively examine how the relative abundances of individual components vary with retention time (RT). |
For Intact Mass Analysis experiments of antibody-drug conjugates (ADCs), calculate the drug load for each component in the analysis and determine the overall drug-to-antibody ratio (DAR) value for the experiment. | |
Compare spectra from different experiments in a mirror plot to note differences and similarities in the structures and relative abundances of components across experiments. | |
Export results as an intact workbook | Save all or selected component results as a specially-formatted intact workbook for use with the Chromeleon chromatography data system. |
Prepare and customize reports | When connected to the Thermo Scientific Ardia Platform, create and share fully customizable data reports for analysis and quality control. |