The following table describes the parameters in the Assign Variable Modifications pane in the Oligonucleotide Sequence Editor.
Parameter | Description |
|---|---|
Max # Modifications area | Displays the maximum number of variable modifications to assign to the oligonucleotide sequence. Range: 0 to 8; default value: 1 |
Terminal Truncation Search area (Visible for oligonucleotide sequences with the "Intact Deconvolution" category only) | |
Enabled Yes/No (buttons) | Enables or disables the Terminal Truncation Search feature for Intact Mass Analysis. Options: Yes or No (default) |
Specificity | Specifies if the truncated terminal is removed from the 5'- or 3'-terminal of the oligonucleotide sequence.
|
Lower Mass Limit | Defines the lower mass limit for the truncated terminal. Range: 0 to 100,000; default value: 1,000 |
Modifications/Modifications Selected for Search area | |
Modifications (left side panel) | Lists the 5'-terminal, 3'-terminal, and building block modifications available to assign to the oligonucleotide sequence. |
Modifications Selected for Search (right side panel) | Lists the 5'-terminal, 3'-terminal, and building block modifications currently assigned to the oligonucleotide sequence. |
Mono. Mass | Displays the monoisotopic mass of the selected modification. |
Avg. Mass | Displays the average mass of the selected modification. |
Residues (Oligo Variable Modification area) | Displays the letter code(s) for the building block(s) associated with the selected modification. |
The following table describes the commands in the Assign Variable Modifications pane in the Oligonucleotide Sequence Editor.
Button | Description |
|---|---|
Add | Adds the selected modification to the Modifications Selected for Search list. |
Remove | Removes the selected modification from the Modifications Selected for Search list. |
Load Default Mods | Quickly loads the (editable) default sublist of modifications into the Modifications Selected for Search list. |