TIP

For Intact Mass Analysis experiments, you can create a mass-only or formula-only oligonucleotide sequence.

Mass-only or formula-only sequences are useful when you want the application to identify components (such as impurities) with a known mass or formula in an Intact Mass Analysis experiment.

NOTE

Mass-only and formula-only sequences are supported for Intact Mass Analysis experiments of oligonucleotides only; these sequences must be assigned the "Intact Deconvolution" category in the Oligonucleotide Sequence Editor.

Procedure

  1. On the BioPharma Finder Home page, in the Experiment Types pane, select the Sequence Manager link.
  2. The Sequence Manager opens.
  3. On the right side of the Sequence Manager, select the New Oligonucleotide button.
  4. The Oligonucleotide Sequence Editor opens.
  5. Oligonucleotide Sequence Editor
    Figure Oligonucleotide Sequence Editor
  6. On the left side of the Oligonucleotide Sequence Editor, in the Sequence Information pane > Target Oligonucleotide area, enter the following information:
  7. Name: Type the name of the oligonucleotide sequence.
  8. Description: (Optional) Type a description for the oligonucleotide sequence.
  9. Category: Select the "Intact Deconvolution" category from the dropdown menu.

Procedure

  1. Sequence Information pane input for a formula-only oligonucleotide sequence
    Figure Sequence Information pane input for a formula-only oligonucleotide sequence
  2. Do either of the following:
  3. To create a formula-only sequence, enter the chemical formula of the sequence in the Formula box, and then select Apply.

  4. NOTE

    For a formula-only oligonucleotide sequence, the maximum number of each element in the chemical formula is 999,999,999.

  5. The application automatically calculates and displays the Monoisotopic Mass and Average Mass parameters for the sequence from the user-specified formula.
  6. To create a mass-only sequence, enter the Monoisotopic Mass and Average Mass parameters for the sequence in the respective boxes.
  7. The Apply button is not available for a mass-only sequence.
  8. Save the oligonucleotide sequence.
  9. The application adds the saved sequence to the Sequence Manager table.
  10. Formula-only oligonucleotide sequence added to the Sequence Manager
    Figure Formula-only oligonucleotide sequence added to the Sequence Manager

IMPORTANT

You cannot assign variable modifications to a mass-only or formula-only oligonucleotide sequence.