Assign modifications to a protein sequence - BioPharma Finder Software - Intact Mass Analysis User Guide - Assign modifications to a protein sequence - Create protein sequences - BioPharma Finder Software - BioPharma Finder Software

1

Disulfide links

Create intra- and inter-chain links between cysteine (C) residues in a protein.

You can assign disulfide links to sequences with the "Intact Protein" category only. The feature is disabled for all other categories.

• Intact Mass Analysis (Intact Protein)

2

Static modifications

Define side-chain or terminal modifications at a single site or all sites for a specified residue or terminal.

• Peptide Mapping Analysis (Peptide Mapping)
• Intact Mass Analysis (Intact Protein)

3

Glycosylations

Specify attachment of sugar moieties at sites in the protein to form N- and O-linked glycans.

• Peptide Mapping Analysis (Peptide Mapping)
• Intact Mass Analysis (Intact Protein)

4

Variable modifications

Define possible side-chain or terminal modifications whose specific sites and number of occurrences might not be known.

• Peptide Mapping Analysis (Peptide Mapping)
• Intact Mass Analysis (Intact Protein)

N/A

Generate proteoforms

Select a sublist of variable modifications to generate a list of proteoforms for searching to identify fragment ions.

You can generate proteoforms for sequences with the "Top Down" category only. This feature is disabled for all other categories.

• Top Down Analysis (Top Down)