This symptom may be due to incomplete desalting or failure of the Dionex ERD 500 component membranes.
Eluent salts result from incomplete desalting, and regenerant salts result from leakage of regenerant through the cation exchange membranes separating the regenerant and eluent chamber.
See the following table for causes of eluent salts in the purified sample.
Issue | Solution |
---|---|
Excessive eluent concentration (i.e., > 0.35 M Na+) | Procedure
|
Excessive flow (i.e., > 1.0 mL/min for 4 mm or > 0.25 mL/min for 2 mm) | |
Inadequate recovery time after exposure to a high concentration of eluent cations | Procedure
|
Inadequate regeneration capacity | This might result from low regenerant flow, low current supply to the Dionex ERD 500, or failure of the Dionex RFC-10 controller or Dionex ERD 500. Procedure
If the Dionex RFC-10 controller or ERD 500 fail, they will need to be replaced. |