The following regeneration conditions allow removal of ≥ 99.5% of the Na+ ion when the eluent Na+ concentration does not exceed 0.35 M as NaOH and NaOAc combined, and eluent flow is ≤ 1.0 mL/min for 4 mm or ≤ 0.25 mL/min for 2 mm.
- 4 mm: 150 mM trifluoroacetic acid flowing at ≥ 7 mL/min RFC-10 at highest setting (500 mA).
- 2 mm: 150 mM trifluoroacetic acid flowing at ≥ 2 mL/min RFC-10 at 125 mA.