Possible cause

Solution

Column overloading

Dilute the sample (see Dilute samples).

Liquid leaks

Locate and eliminate any leaks (see Liquid Leaks).

Incomplete or imprecise filling of sample loop

Procedure

  1. Fill the sample loop until excess sample exits the waste line.
  2. Inspect the 1-cc syringe (P/N 016388) for damage. If necessary, replace the syringe.

Pump not primed properly

Prime the pump (see Prime the analytical pump).