Items needed:
- Dionex CRD 200, 2 mm (P/N 062986)
- 5 cc disposable plastic syringe (P/N 016640)
- 10-32 luer adapter (P/N 046888)
- Degassed DI water
Procedure
- Place the required items on a clean work surface near the Dionex Inuvion IC System. Unbox the Dionex CRD and remove the plugs and any other packing material.
- To hydrate the Dionex CRD, push approximately 3 mL of degassed DI water through the ELUENT IN port, using the 5 cc syringe and 10-32 luer adapter.
- Discard the effluent from the ELUENT OUT port into the waste beaker.
- Using the 5 cc syringe, push 5 mL of degassed water through the REGEN IN port.
IMPORTANT
Use gentle (but steady) pressure to prevent damage to the membrane of the Dionex CRD.
- Discard effluent from the REGEN OUT port into the waste beaker.
- Wait approximately 10 minutes to fully hydrate the membrane in the Dionex CRD.
- Then, connect the suppressor ELUENT OUT to the Dionex CRD ELUENT IN.
- Place the Dionex CRD in the holder to the left of the suppressor.
- Connect the CRD ELUENT OUT to the conductivity cell INPUT.
- Direct the CRD REGEN OUT to waste.
- Connect the CRD REGEN IN to the suppressor REGEN OUT.