FAQ Task Table

Question

Troubleshooting

How do I connect an autosampler to my Dionex Inuvion IC System?

For instructions on how to connect the system to a Dionex AS-AP, Dionex AS-DV, or Dionex AS-HV, refer to the autosampler operator’s manual.

How do I print?

In the Chromeleon CDS Console, select Print from the Instrument toolbar above the ePanel Set.

Why are the retention times moving?

Retention times can shift if the pump flow is erratic, or if either the column or eluent is contaminated.

  1. For pump flow rate troubleshooting, see Erratic flow and/or pressure reading.
  2. Clean the column as instructed in the column manual. If this does not eliminate the problem, replace the column.
  3. If eluent is manually degassed or is sensitive to contamination, Thermo Fisher Scientific recommends pressurizing the bottle with helium or nitrogen. For more information, see Set up the eluent bottle.

How do I adjust retention times?

Retention times are calculated during calibration. The Use Recently Detected Retention Time parameter in the Chromeleon CDS Processing Method Editor can be used to compensate for some types of retention time drift, such as evaporation of volatile components in pre-mixed solvents or an aging column. For details, refer to the Chromeleon CDS Help.

When should I remake standards?

Standards are used only for calibration and should always be freshly made due to their limited lifetime of one week.

When should I replace the eluent generator cartridge?

The Dionex Inuvion RFIC monitors Dionex EGC use. When the cartridge needs replacement, a message is displayed in the Chromeleon CDS audit trail. To view the remaining cartridge life, select the EG Settings on the Inuvion ePanel.

How do I start Chromeleon CDS?

  1. Select Start on the Windows taskbar.
  2. Select All Programs > Thermo Chromeleon 7 > Chromeleon 7.

How do I delete data?

  1. In the Chromeleon CDS Console, on the Navigation Pane, select the Data Category Bar.
  2. In the data tree, right-click the sequence and select Delete.

How do I back up data?

Refer to the “Backup Procedure” and “Data File Locations” topics in the Chromeleon CDS Help.

How do I shut off the system?

Turn off the power switch on the Dionex Inuvion IC System rear panel (see Dionex Inuvion IC System rear panel).

For longer shutdowns (over a week), Thermo Fisher Scientific recommends flushing the system with deionized water if the system was using manually prepared eluent.

To flush the system before an extended shutdown:

  1. Connect the system to a bottle containing deionized water.
  2. Bypassing all consumables, flush the system through the pump head and detector cell directly to waste.

How do I store columns?

Columns should be stored in eluent. Refer to the column manual for complete instructions.

How do I know when a column is dirty?

Refer to the troubleshooting section of the column manual.

How do I clean a column?

Refer to the troubleshooting section of the column manual.

Why is the conductivity high?

Possible reasons for high conductivity include:

  1. The suppressor is not on. Turn on the suppressor from the Inuvion ePanel in Chromeleon CDS.
  2. The suppressor needs regeneration. For troubleshooting guidance, refer to the suppressor manual.
  3. For additional troubleshooting information, see High cell output.

How do I configure and operate the auxiliary valve?

The auxiliary valve can be installed by users or by Thermo Fisher Scientific service engineers.

For details about valve operation, see Auxiliary valve (optional). For maintenance details, see User maintenance.