Before putting the new suppressor to use, we recommend measuring the backpressure to the suppressor, ensuring that the suppressor is not exposed to high backpressure inadvertently. Do not exceed 100 psi of backpressure to the suppressor (150 psi for 2 mm anion versions). The backpressure to the suppressor comes from any tubing, detector cell, backpressure coils, or any other components that are fluid-connected to the eluent out port of the suppressor.
NOTE
Hydrating the suppressor ensures that the ion exchange materials are in a swollen form for proper operation. A 20-minute static step is recommended during first time installation or after long suppressor idle time to ensure complete hydration. Failure to follow proper hydration protocols may result in leakage upon installation.
Use the following checklist to measure the backpressure to the suppressor.
Step 1: Disconnect the column out/eluent in tubing from the old suppressor and direct it to waste. Turn on the pump at the application flow rate for 10 minutes. Record the system pressure 1 (P1) and turn off the pump. | □ | |
P1, ________ psi | □ | |
Step 2: Connect the column out/eluent in tubing to the detector cell inlet, remove the backpressure coils after the detector cell if installed, and direct the detector cell outlet tubing to waste. Turn on the pump at the application flow rate for 10 minutes. Record the system pressure 2 (P2) and turn off the pump. | □ | |
P2, _________psi | □ | |
Calculate the backpressure across the detector cell (BP1): BP1 = P2 – P1 = ________psi The backpressure of the detector cell is typically ≤ 70 psi at a flow rate of 2 mL/min. If the backpressure exceeds this specification, see Detector Cell Backflush Section | □ | |
Step 3: Connect the recommended number of backpressure coil(s) (see table below) to the detector cell outlet and direct the backpressure coil outlet to waste. Turn on the pump at the application flow rate for 10 minutes. Record the system pressure (P3) and turn off the pump. | □ | |
4 mm Chromatography P/N: 045877 1 Coil = 1.5 – 3.0 mL/min 2 Coils = 0.5 – 1.5 mL/min | ||
2 mm Chromatography P/N: 045878 1 Coil = 0.37 – 0.75 mL/min 2 Coils = 0.12 – 0.37 mL/min | ||
P3, ________ psi | □ | |
Calculate the backpressure across the backpressure coil(s) (BP2): BP2, = P3 – P2 = ________ psi Pass: 30-40 psi for carbonate eluent or 10-40 psi for other eluents
If the back pressure exceeds the specification, see Assemble back pressure coils. | □ | |
Before putting the new suppressor to use, hydrate it properly first.
CAUTION
Improper hydration will result in irreversible damage to suppressor and will void the warranty.
Use the following checklist to hydrate and check the backpressure of the suppressor.
Step 4: Connect the column out tubing to the suppressor Eluent Inlet and direct the suppressor Eluent Outlet to the Regen Inlet of the suppressor using ~ 6” of green PEEK tubing (ID 0.030”) provided with the system ship kit. Direct the suppressor Regen Outlet to waste (see the following figure). | □ |
Turn on the pump and flow ≤10 mM eluent or DI water at 0.5 mL/min for 4mm suppressor or 0.125 mL/min for 2mm suppressor for 20 minutes. The power to the suppressor must be turned off during this step. | □ |
Allow the suppressor to rest undisturbed for 20 minutes to fully hydrate the resin, screens, and membranes. | □ |
Turn on the pump at the application flow rate for 10 minutes; record the system pressure 4 (P4) and turn off the pump. |
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P4, _______ psi | □ |
Calculate the backpressure across the suppressor (BP3): BP3 = P4 – P1 = ________ psi Pass: ≤ 200 psi
If the backpressure exceeds this specification, see Suppressor Backflush with DI Water section
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Step 5: Connect the suppressor Eluent outlet tubing to the detector cell inlet. Then connect the detector cell outlet tubing with backpressure coil(s) to the Regen Inlet of the suppressor, as shown in Figure 2, for the Auto Suppression Recycle Mode Configuration. For External Water Mode Configuration, refer to the suppressor manual for detailed installation guide. | □ |
The suppressor is now ready to be installed and configured (see the following figure). Normal operation using the application eluent strength, flow rate, and recommended current/voltage power settings can be applied. | □ |