Procedure
- Turn off the suppressor power and system pump.
- Disconnect the analytical (and guard) column(s) from the injection valve and the Dionex DRS 600, Dionex ERS 500e or Dionex AERS 500 Carbonate. Refer to the specific analytical column Product Manual for column cleanup procedures.
- If you are running in the Auto Suppression External Water Mode, turn off the external water and disconnect the external water line from the suppressor REGEN IN port.
- Disconnect the liquid line from the suppressor ELUENT OUT port to the cell at the cell fitting and reconnect it to the REGEN IN port.
- Connect a temporary line from the priming block or the low-pressure tee on the isocratic or gradient pump to a container with a solution of freshly prepared 10% 1.0 N methane sulfonic acid or sulfuric acid and 90% acetonitrile or methanol. Acid/acetonitrile solutions are not stable during long-term storage, so this cleanup solution must be made immediately before each column cleanup. Alternatively, it can be proportioned from 1 bottle containing 1.0 N acid and another bottle containing 100% acetonitrile. For 4 mm systems pump this solution through the suppressor at 1 – 2 mL/min for 30 minutes. For 2 mm systems, pump this solution through the suppressor at 0.25–0.50 mL/min for 30 minutes.
NOTE
Bypassing internal pump manifolds when temporarily pumping high concentration cleaning solutions significantly reduces the time required to re-equilibrate the system to low concentration eluents.
- Flush the suppressor with deionized water for 10 minutes.
- Reinstall the analytical (and guard) column(s). Begin pumping eluent through the system at the flow rate required for your analysis and equilibrate the system and resume normal operation.