Without Guard
- Eluent 1: Type I DI Water
- Eluent 2: 5.0 mM NaOH
- Eluent 3: 100 mM NaOH
- Eluent Flow Rate: 2.0 mL/min
TIME (min) | % E1 | % E2 | %E3 | Comments |
|---|---|---|---|---|
Equilibration | ||||
0 | 90 | 10 | 0 | 0.5 mM NaOH for 7 min |
7.0 | 90 | 10 | 0 | |
Analysis | ||||
0.0 | 90 | 10 | 0 | 0.5 mM NaOH, Inject |
0.2 | 90 | 10 | 0 | Inject Valve to Load Position |
2.0 | 90 | 10 | 0 | 0.5-5.0 mM NaOH in 3 min |
5.0 | 0 | 100 | 0 | 5.0-38.25 mM NaOH in 10 min |
15.0 | 0 | 65 | 35 | |
With Guard
- Eluent 1: Type I DI Water
- Eluent 2: 5.0 mM NaOH
- Eluent 3: 100 mM NaOH
- Eluent Flow Rate: 2.0 mL/min
TIME (min) | % E1 | % E2 | %E3 | Comments |
|---|---|---|---|---|
Equilibration | ||||
0 | 90 | 10 | 0 | 0.5 mM NaOH for 7 min |
7.0 | 90 | 10 | 0 | |
Analysis | ||||
0.0 | 90 | 10 | 0 | 0.5 mM NaOH, Inject |
0.2 | 90 | 10 | 0 | Inject Valve to Load Position |
2.5 | 90 | 10 | 0 | 0.5-5.0 mM NaOH in 3.5 min |
6.0 | 0 | 100 | 0 | 5.0-38.25 mM NaOH in 12 min |
18.0 | 0 | 65 | 35 | |
NOTE
The steps for sample neutralization can be performed while the Thermo Scientific Dionex IonPac AS11 is equilibrating with the starting eluent (90% E1/10% E2).
Seven minutes are required at the beginning of the above program for equilibration of the Dionex IonPac AS11 with E1 prior to injecting the next sample.
If the system is not used continuously, that is, the run program (equilibration plus analysis) is not started exactly every 22 minutes (without Dionex IonPac AG11) or 25 minutes (with Dionex IonPac AG11), the run program can be modified to start with 2 minutes of the highest eluent concentration for regeneration and then to equilibrate with E1 for 7 minutes with the next injection 9 minutes into the program.