A sequence determines how a group of injections is processed. The Injection list (see Injections) is the primary element of a sequence. This list groups injections in the order of processing and includes injection variables (name, type, and so on) that characterize each injection. Various data items can be included in a sequence.

You can create a sequence in the Chromeleon Console, from the injection queries, from LIMS worklists, or by using an eWorkflow (see Creating a sequence).


For more information about different options for creating sequences, refer to the Editing Sequences and Injection Lists: Create a Sequence topic in the Chromeleon 7 Help.

A sequence can only have one instance of the following types of data:

  • Injection list: A group of injections that are analyzed in the order in which they are listed. A sequence can only have one injection list.
  • Injection results: The chromatographic data acquired for each injection, as defined by the injection list.
  • Assigned Instrument: Each injection can have a different instrument method, but only one instrument can be assigned to a sequence at a time.
  • Electronic Report: The results of a sequence that are stored as an electronic printout copy associated with the sequence. The electronic report lets you sign the results of a sequence with an electronic signature.

A sequence can have multiple instances of the following types of data:

  • Instrument method: The commands used to control an instrument when it acquires data.
  • Processing method: The parameters used to evaluate the injection results.
  • View settings: The settings used to display data in the Chromatography Studio.
  • Report template: The template used to print injection results.
  • Spectral library: A group of spectra used to identify unknown substances.

To create a sequence using the Sequence Wizard, follow these steps:


  1. Under the Instruments category in the Chromeleon Console, select the instrument that you want to create a sequence for from the navigation pane.
  2. From the Create menu, select Sequence.
  3. Follow the on-screen instructions to complete each page of the Sequence Wizard, and then select Finish.
  4. Select the location for the new sequence, enter a name, and then select Save.
  5. The new sequence is displayed in the work area of the Chromeleon Console.

To create a sequence using the Sequence Wizard in the Chromeleon Console, the following data files must be prepared: