The following table lists defects that were resolved or no longer relevant between the BioPharma Finder 1.0 and BioPharma Finder 5.4 software release, excluding Help issues and cosmetic fixes. In some cases, the description has been amended or extended from the original version to better describe the reported issue. The resolution of each of the listed issues involves both an engineering fix and follow-up testing verified by our product evaluation department.

Table Resolved issues in BioPharma Finder

Item number

Software area

Description

240503

Ardia Platform

A network error prevents multiple users from opening the same experiment simultaneously.

397422

Ardia Platform

After leaving the BioPharma Finder application running overnight, the Ardia homepage appears, instead of the expected login dialog box.

554126

Ardia Platform

Ardia Platform version 1.0 is not compatible with BioPharma Finder 5.3.

639912

605057

Ardia Platform

Reports saved for Peptide Mapping Analysis experiments in Ardia Platform version 1.1 are not displayed in BioPharma Finder versions 5.1 or 5.2.

38723

General

Application updated to integrate the latest version of MongoDB.

38744

General

Memory usage is not optimized during various operations, such as loading results or switching from the Process and Review page to the Mapping page.

(TFS) 40758

Installer

MsFileReader is not installed when running the installer in repair mode.

(TFS) 81000

Installer

On a few Windows 7 machines, when the BioPharma Finder installer is run, the installer might get stuck at the "Initializing" step.

123001

Installer

Attempting to upgrade to BioPharma Finder to version 4.0 (or later) will fail if the user has not launched the current version (3.0 or earlier) of the software.

(TFS) 3388

Intact Mass Analysis

When a user resets a processing method, the chromatogram parameters are not reset.

(TFS) 5145

Intact Mass Analysis

If a user zooms in on a chromatogram, the modification peak labels do not update when selecting another peak.

(TFS) 23543

Intact Mass Analysis

When 64-bit ReSpect™ DLL is delivered with BPF 1.0, the data intensity is very different and missing some low-resolution data.

39286

Intact Mass Analysis

When BioPharma Finder version 1.0sp1 is upgraded to 2.0, the retention time (RT) range for custom processing methods is set to “0” instead of the correct range.

39311

Intact Mass Analysis

On the Parameters > Report page of the processing method editing wizard, all of the reporting parameters are shown as “enabled” for a multiconsensus experiment with all reporting parameters disabled.

39526

Intact Mass Analysis

On the Reporting page, the header in the “Source Chromatogram” section displays “F:” for filter, but filters do not apply to chromatograms.

39534

Intact Mass Analysis

For an Xtract with Sliding Windows deconvolution experiment, when you save a component as a reference from the Process and Review > Results table, the information displayed for the reference spectrum incorrect.

39535

Intact Mass Analysis

On Parameters > Target Sequence Matching page of the processing method editing wizard, the default “Sequence Matching Tolerance” parameter is very large; this parameter has been updated to a reasonable value of 20 ppm.

39537

Intact Mass Analysis

On the Process and Review page, after zooming out to full-scale on the source spectrum or deconvoluted spectrum, the scroll bar is still visible.

39538

Intact Mass Analysis

When a user exports the top-level results in the Process and Review > Results table, the exported file contains an additional expandable row (blank) after the last component row.

39540

Intact Mass Analysis

On the Reporting page, the order of information listed in the “Matching Sequence” column of the “ReSpect/Xtract Masses Table” section is not consistent with the order displayed in the “Deconvolved Spectrum” section or in the Process and Review > Results table.

39541

Intact Mass Analysis

When a user selects a component in the Process Review > Results table, the chromatogram displays the zoomed retention time (RT) range from the previously selected component instead of resetting the full-scale chromatogram view.

39542

Intact Mass Analysis

In an uncommon scenario involving an Xtract deconvolution, the run queue becomes corrupted and the experiment fails.

39543

Intact Mass Analysis

If the user adds a reference spectrum to an Xtract with Sliding Windows deconvolution experiment, the experiment fails.

39545

Intact Mass Analysis

During an Xtract deconvolution, experiment processing fails intermittently due to a missing source spectrum.

39546

Intact Mass Analysis

The application takes an excessive amount of time to add a large number of jobs to the run queue.

39558

Intact Mass Analysis

The extracted ion chromatogram (XIC) is generated incorrectly for experiments with both full-scan and MS/MS data after the ReSpect or Xtract deconvolution.

39559

Intact Mass Analysis

A warning message has been implemented to warn users that the ReSpect or Xtract algorithms cannot deconvolve centroid data, only profile data.

40004

Intact Mass Analysis

There are several UI style inconsistencies in Protein Deconvolution 4.0.

40019

Intact Mass Analysis

On the Parameters > Chromatogram page of the processing method editing wizard, if invalid values are entered for the “Chromatogram m/z Range” parameter, the expected red error message on the communicator bar does not appear.

40409

Intact Mass Analysis

After copying and pasting the source spectrum into a third-party application, the peak markers are not correctly aligned with the peaks in the pasted image.

45964

Intact Mass Analysis

In the Process and Review > Results table, the “Apex RT” value displayed for some components is outside of the range indicated by the “Start Time (min)” and “Stop Time (min)” values.

119790

Oligonucleotide Analysis

In the Oligonucleotide Sequence Editor, entering a sequence with a formula that contains any elements with a quantity greater than 100,000 results in an error.

123294

Oligonucleotide Analysis

In the Predicted Oligo MS/MS (Kinetic Model) feature, when a sequence is entered using lower case letters, no warning message is displayed stating that the sequence should be entered in capital letters or triplet code format.

(TFS) 14263

Peptide Mapping Analysis

When a protein coverage map is copied and pasted to Microsoft Office 2007, some amino acids are not displayed properly.

(TFS) 16720

Peptide Mapping Analysis

Disulfide bonds are not properly highlighted on the Protein Sequence tab.

(TFS) 20965

Peptide Mapping Analysis

The sequence coverage map does not paste into Microsoft Word™ correctly.

(TFS) 24538

Peptide Mapping Analysis

Peptides might not be properly highlighted on the Protein Sequence tab.

(TFS) 38277

Peptide Mapping Analysis

A refresh issue occurs when loading a protein sequence after reprocessing an experiment.

38385

Peptide Mapping Analysis

If a peptide has more than one modification, the peptide is excluded from the “% Relative Abundance” calculations displayed on the Mapping > Modification Summary page.

38396

Peptide Mapping Analysis

For experiments with a single raw data file, the “ID Type” value displayed for some components in the Processing and Review > Results table is “Full”, even though the components have an associated MS2 spectrum.

38707

Peptide Mapping Analysis

Processing a large experiment results in the application using only one raw data file for the MS2 spectrum.

38708

Peptide Mapping Analysis

The application selects the activation type as ETD over higher-quality CID in the MS2 spectrum.

38733

Peptide Mapping Analysis

In an experiment with multiple raw data files, the file-specific “Avg MS Area” and “%CV” columns in the Process and Review > Results table are displayed out of order.

39285

Peptide Mapping Analysis

The protein coverage results in the Mapping > Coverage > Results table might not agree with those displayed on the Protein Coverage Map.

39305

Peptide Mapping Analysis

Double-clicking on a completed experiment in the run queue does not open the experiment results.

39309

Peptide Mapping Analysis

The application might crash if the user cancels an actively processing experiment.

39324

Peptide Mapping Analysis

In the Process and Review > Results table, the information in the “Modification” column is displayed incorrectly for a component with seven modifications.

39371

Peptide Mapping Analysis

In the Process and Review > Result table, glycosylated peptides are not identified when the user defines an N-glycan as a variable modification in the protein sequence.

40106

Peptide Mapping Analysis

The data resolution displayed in the MS2 spectrum on the Process and Review page might be incorrect.

40109

Peptide Mapping Analysis

Toggling between fragmentation types (Auto/CID/ETD) in the MS2 spectrum on the Process and Review page results in scaling and data label discrepancies between spectra.

40197

Peptide Mapping Analysis

For an experiment using the “Find All Ions with MS/MS” task specified on the Parameters > Component Detection page of the processing method editing wizard, the Modification Results table on the Mapping > Modification Summary page is blank.

40200

Peptide Mapping Analysis

Isomerization of the same peptide component shows up as two different modification masses with a large ∆ ppm value in the Process and Review > Results table.

(TFS) 40794

Peptide Mapping Analysis

When the MS2/full scan spectra window is changed to "floating" by dragging the window and then changed back to "dockable," the data is not displayed anymore.

52377

Peptide Mapping Analysis

In the Process and Review > Results table, the “Theoretical Mass” value does not include Na+ adducts.

52382

Peptide Mapping Analysis

In the Process and Review > Results table, the “Theoretical Mass” value does not include K+ adducts in the mass.

52383

Peptide Mapping Analysis

In the Process and Review > Results table, the “Theoretical Mass” value does not include glycans in the mass.

52830

Peptide Mapping Analysis

In the Process and Review > Results table, the “Theoretical Mass” value is incorrect for dimeric peptide components.

52831

Peptide Mapping Analysis

In the Process and Review > Results table, the “Theoretical Mass” value does not include oxidation for gas phase oxidation in the mass.

52946

Peptide Mapping Analysis

In the Modification Summary, the “Predicted Time” value is not always the same when processing the same experiment multiple times.

78060

Peptide Mapping Analysis

When a user exports checked components in the Process and Review > Results table “As Displayed”, the columns in the exported table overlap and are misaligned.

138669

Peptide Mapping Analysis

For a targeted experiment, the “ID Type” values displayed in the Process and Review > Results table is incorrect; components with a “Full” ID type have associated fragment coverage maps.

150046

Peptide Mapping Analysis

For host cell protein (HCP) experiments, performance issues could occur when searching extremely large data sets.

257333

Peptide Mapping Analysis

Isomerization does not appear as a modification in the Result table.

39378

Protein Sequence Editor

The chain mass is not updated after linking disulfide bonds in the Protein Sequence editor.

230105

Reporting

For experiments with large data files, shading might not be visible when a chromatogram is added to a report.

39539

Run queue

After processing a job, the run queue table displays the bottom of the job list.