Key Features

The BioPharma Finder 5.4 application provides a comprehensive suite of workflows designed to enable and simplify biotherapeutic attribute characterization. Driven by a wide array of advanced algorithms developed to fit the specific needs of biotherapeutic drug development, in-depth data analysis is streamlined and automated for fast and simple data interpretation. In the latest release, BioPharma Finder integrates new features to support the advanced fragmentation modes available in the latest portfolio of Thermo Scientific mass spectrometers. Specifically, the application supports electron transfer/high-energy collision dissociation (EThcD) fragmentation, allowing the identification of and distinction between positional isomers Leu/Ile and Asp/iso-Asp. Additionally, in the Top Down Analysis workflow, BioPharma Finder 5.4 supports MS2 data acquired on Thermo Scientific Orbitrap Astral instruments.

New to BioPharma Finder 5.4

The following features are new to BioPharma Finder 5.4:

  • Support for EThcD fragmentation
  • Orbitrap™ Excedion™ Pro adds the ETD & EThcD fragmentation types to the Orbitrap Exploris™ series of instruments. This update allows BioPharma Finder to read the new instrument and fragmentation types to process and interpret data acquisition from this instrument.
  • Support distinction of D/IsoD based on EThcD fragmentation
  • Users can confirm the presence of IsoD residues through identification of the presence unique ions (z-57 and c+57) generated by EThcD fragmentation. The application analyzes the fragmentation spectra and annotates the corresponding ions for peptides with IsoD.
  • Support distinction of L/I based on EThcD fragmentation
  • Users can discriminate between leucine and isoleucine in peptides analyzed using EThcD fragmentation. The application provides clear annotations of the unique fragment ions generated by EThcD fragmentation for each of the respective residues (z-29 for isoleucine and z-43 for leucine).
  • Isotopic distribution plot for Xtract deconvolution
  • Users can view the deconvoluted spectrum as an isotopic distribution plot when using Xtract deconvolution the Intact Mass Analysis workflow.