The second page of the processing method editing wizard is the Identification page. Use the Identification page to add or edit protein sequences and then specify which ones to add to the top down analysis experiment for processing.

Furthermore, for experiments containing full-scan MS data with the Intact Deconvolution option enabled, you can define the mass tolerance for target sequence matching and set the merge options for the multiconsensus result format.

Prerequisites

  1. For a top down analysis experiment, you entered an experiment name, loaded the raw data files and entered the conditions (if applicable), and selected a protein sequence on the Top Down Analysis page. You are editing the processing method using the wizard.

Procedure

  1. To open the Identification page, do one of the following:
  2. On the Component Detection page, select Next on the command bar.
  3. Select the Parameters tab on the navigation bar and then select the Identification subtab.
  4. The Identification page contains up to four sections containing editable parameters: Sequence Matching Mass Tolerance, Multiconsensus Component Merge, Sequences Added to Experiment (table), and Global Sequence Reference (table).
  5. See Identification page tables.
  6. image/svg+xml Navigation bar Sequencematchingparameter Multiconsensusmergeparameters
    Identification page parameters (left side)
  7. The Sequence Matching Mass Tolerance and Multiconsensus Component Merge areas are active only if you select the Intact Deconvolution checkbox for at least one of the peaks under the Component Detection > Peaks page because the application uses these parameters only for processing experiments containing full-scan MS data.
  8. These sections are inactive for experiments containing MS2 data or experiments containing full-scan MS data with the Intact Deconvolution option disabled.
  9. image/svg+xml Command bar
    Identification page tables (right side)
  10. (For experiments containing full-scan MS data with the Intact Deconvolution option enabled) Enter the appropriate parameter values in the Sequence Matching Mass Tolerance and Multiconsensus Component Merge areas.
  11. See Identification page parameters.
  12. As needed, you can add, delete, or edit sequences using the Sequences Added to Experiment and Global Sequence Reference tables.
  13. See Sequences for top down analysis experiments.
  14. When you are finished editing the parameters on the Identification page, select Next on the command bar to advance to the Save Experiment page.