In the BioPharma Finder application, top down analysis is similar to intact mass analysis in the following ways:

  • The application extracts the averaged mass spectra, deconvolves the isotopically unresolved or resolved mass of the proteins, and then generates results.
  • The application utilizes two deconvolution algorithms for mass spectral data: Xtract and ReSpect.
  • NOTE

    Top down analysis currently supports both the Xtract and ReSpect deconvolution algorithms for full-scan MS data processing.

    For MS2 data, only Xtract is supported.

  • When you run an experiment with multiple raw data files, you can select either the Batch Processing or Multiconsensus format for your results.
  • You can set up one or more protein sequences and select them for target sequence matching when processing full-scan MS data.