The following table describes the data in the table in the ProSightBP Output pane.

ProSightBP Output table columns

Column

Description

Fragment ion level

+/-

Select to show or hide the lower level of charge state information related to the currently selected fragment ion row.

Row number (bolded)

The number assigned to each visible fragment ion row in the table. This sequential numbering does not change when you sort or filter the table.

(Check box)

Selects/deselects a fragment ion row.

Select the checkbox for an ion fragment row if you want to export the results in the row. See Export the ProSightBP Output Results table.

To select/deselect the checkboxes for all fragment ion rows at once, select or clear the checkbox in the column header.

If you filter the table, the following occurs:

  • In a filtered view, selecting all checkboxes affects only the filtered (currently visible) component rows.
  • In a filtered view, deselecting all checkboxes affects all the component rows in the table, currently visible or not.

Ion Name

Displays the name of a fragment ion in the format of a letter followed by a number, for example "C24".

The letter "C" represents the ion type and the number "24" represents the ion number.

If the fragment ion is not identified, this cell is empty.

Monoisotopic Mass

Displays the observed monoisotopic mass for an individual fragment ion that results from the Xtract deconvolution algorithm.

Sum Intensity

Displays the sum of the intensities of the isotopic clusters for an individual fragment ion that results from the Xtract deconvolution algorithm.

Relative Abundance

Displays the relative abundance for a fragment ion.

The application assigns the most abundant fragment ion (the largest value in the Sum Intensity column) in a deconvoluted spectrum a relative abundance of 100 percent.

The number in the Relative Abundance column represents the Sum Intensity value for a particular fragment ion divided by the largest value in the Sum Intensity column, multiplied by 100.

For example, if the most intense fragment in a deconvoluted spectrum has an intensity of 1000, the application assigns it a relative abundance of 100 percent. If the next most abundant peak has an intensity of 500, the application assigns it an abundance of 50 percent.

Ion Type

Displays the ion type as a letter, as shown in the Ion Name column.

If the fragment ion is not identified, this cell is empty.

Ion Number

Displayed as a number, as shown in the Ion Name column.

If the fragment ion is not identified, this cell is empty.

Theoretical Mass

Displays the theoretical mass returned from the ProSight Lite application for an individual fragment ion

If the fragment ion is not identified, this cell is empty.

Corrected Delta Mass (Da)

Displays the difference between the observed and theoretical masses of a fragment ion, measured in Da.

If the fragment ion is not identified, this cell is empty.

Corrected Delta Mass (ppm)

Displays the difference between the observed and theoretical masses of a fragment ion, measured in ppm.

If the fragment ion is not identified, this cell is empty.

Number of Charge States

Displays the number of charge states for an individual fragment ion that results from the Xtract algorithm.

Charge State Distribution

Displays the range of charge states for an individual fragment ion that the Xtract deconvolution algorithm detected, listed from the lowest to the highest charge state.

Start Time (min)

Displays the start of the retention time (RT) range (in minutes) for a particular peak, as entered in the processing method.

Stop Time (min)

Displays the end of the retention time (RT) range (in minutes) for a particular peak, as entered in the processing method.

Raw File Name

Displays the name of the loaded raw data file related to a particular fragment ion mass.

Activation Type

Displays the fragmentation type for a particular peak specified in the processing method.

Charge state level

Row number (bolded)

The number assigned to each visible charge state row in the table.

This sequential numbering does not change when you sort or filter the table.

Charge State

Displays the specific charge state for a selected component.

Charge state refers to the imbalance between the number of protons and the number of electrons in a molecular species (or an ion adduct).

Calculated Monoisotopic m/z

Displays the mass-to-charge ratio of the calculated monoisotopic mass for a specific charge state.

Monoisotopic Mass of Charge State

Displays the detected monoisotopic mass for a specific charge state.

Most Abundant m/z

Displays the mass-to-charge ratio of the most abundant isotope (or the height of the tallest peak in the isotopic distribution).

Charge Normalized Intensity

Displays the ratio of the intensity of a specific charge state divided by the highest intensity charge state for a given fragment ion.

Fit %

Displays the quality of the match between a measured isotope pattern and an averagine distribution of the same mass.

This column displays a value between 0 and 100 percent.

A fit factor of 100% means that the observed peaks in the measured isotope profile are identical to those in a theoretical averagine distribution and that any missing peaks fall below a restrictive threshold.

Fit % Left

Displays the difference between the average mass for a component and the calculated monoisotopic mass for a specific charge state (in Da).

Fit % Right

Displays the difference between the average mass for a component and the calculated monoisotopic mass for a specific charge state (in ppm).