The following table describes the data in the table in the ProSightBP Output pane.
Column | Description |
|---|---|
Fragment ion level | |
+/- | Select to show or hide the lower level of charge state information related to the currently selected fragment ion row. |
Row number (bolded) | The number assigned to each visible fragment ion row in the table. This sequential numbering does not change when you sort or filter the table. |
(Check box) | Selects/deselects a fragment ion row. Select the checkbox for an ion fragment row if you want to export the results in the row. See Export the ProSightBP Output Results table. To select/deselect the checkboxes for all fragment ion rows at once, select or clear the checkbox in the column header. If you filter the table, the following occurs:
|
Ion Name | Displays the name of a fragment ion in the format of a letter followed by a number, for example "C24". The letter "C" represents the ion type and the number "24" represents the ion number. If the fragment ion is not identified, this cell is empty. |
Monoisotopic Mass | Displays the observed monoisotopic mass for an individual fragment ion that results from the Xtract deconvolution algorithm. |
Sum Intensity | Displays the sum of the intensities of the isotopic clusters for an individual fragment ion that results from the Xtract deconvolution algorithm. |
Relative Abundance | Displays the relative abundance for a fragment ion. The application assigns the most abundant fragment ion (the largest value in the Sum Intensity column) in a deconvoluted spectrum a relative abundance of 100 percent. The number in the Relative Abundance column represents the Sum Intensity value for a particular fragment ion divided by the largest value in the Sum Intensity column, multiplied by 100. For example, if the most intense fragment in a deconvoluted spectrum has an intensity of 1000, the application assigns it a relative abundance of 100 percent. If the next most abundant peak has an intensity of 500, the application assigns it an abundance of 50 percent. |
Ion Type | Displays the ion type as a letter, as shown in the Ion Name column. If the fragment ion is not identified, this cell is empty. |
Ion Number | Displayed as a number, as shown in the Ion Name column. If the fragment ion is not identified, this cell is empty. |
Theoretical Mass | Displays the theoretical mass returned from the ProSight Lite application for an individual fragment ion If the fragment ion is not identified, this cell is empty. |
Corrected Delta Mass (Da) | Displays the difference between the observed and theoretical masses of a fragment ion, measured in Da. If the fragment ion is not identified, this cell is empty. |
Corrected Delta Mass (ppm) | Displays the difference between the observed and theoretical masses of a fragment ion, measured in ppm. If the fragment ion is not identified, this cell is empty. |
Number of Charge States | Displays the number of charge states for an individual fragment ion that results from the Xtract algorithm. |
Charge State Distribution | Displays the range of charge states for an individual fragment ion that the Xtract deconvolution algorithm detected, listed from the lowest to the highest charge state. |
Start Time (min) | Displays the start of the retention time (RT) range (in minutes) for a particular peak, as entered in the processing method. |
Stop Time (min) | Displays the end of the retention time (RT) range (in minutes) for a particular peak, as entered in the processing method. |
Raw File Name | Displays the name of the loaded raw data file related to a particular fragment ion mass. |
Activation Type | Displays the fragmentation type for a particular peak specified in the processing method. |
Charge state level | |
Row number (bolded) | The number assigned to each visible charge state row in the table. This sequential numbering does not change when you sort or filter the table. |
Charge State | Displays the specific charge state for a selected component. Charge state refers to the imbalance between the number of protons and the number of electrons in a molecular species (or an ion adduct). |
Calculated Monoisotopic m/z | Displays the mass-to-charge ratio of the calculated monoisotopic mass for a specific charge state. |
Monoisotopic Mass of Charge State | Displays the detected monoisotopic mass for a specific charge state. |
Most Abundant m/z | Displays the mass-to-charge ratio of the most abundant isotope (or the height of the tallest peak in the isotopic distribution). |
Charge Normalized Intensity | Displays the ratio of the intensity of a specific charge state divided by the highest intensity charge state for a given fragment ion. |
Fit % | Displays the quality of the match between a measured isotope pattern and an averagine distribution of the same mass. This column displays a value between 0 and 100 percent. A fit factor of 100% means that the observed peaks in the measured isotope profile are identical to those in a theoretical averagine distribution and that any missing peaks fall below a restrictive threshold. |
Fit % Left | Displays the difference between the average mass for a component and the calculated monoisotopic mass for a specific charge state (in Da). |
Fit % Right | Displays the difference between the average mass for a component and the calculated monoisotopic mass for a specific charge state (in ppm). |