The columns in the Intact Deconvolution Results table on the Process and Review page vary depending on the selected deconvolution algorithm (ReSpect or Xtract) and processing method parameters. These columns are similar for the Results tables produced for intact mass analysis.
For more information, see the following topics in the Intact Mass Analysis User Guide:
- "Results table parameters (for single file or batch format experiments)"
- "Results table parameters (multiconsensus format experiments)"
NOTE
For top down analysis, the BioPharma Finder application uses a deconvolution method for the Xtract and ReSpect algorithms that is similar to the "Average Over Selected Retention Time" method used for intact mass analysis experiments.
Therefore, when viewing the Results table parameters in the Intact Mass Analysis User Guide (linked above) for more information on the Intact Deconvolution Results table for top down analysis, refer specifically to the parameters marked with "Y" (for "yes") in the Xtract > Avg o/RT and ReSpect > Avg o/RT columns.
These parameters are applicable to top down analysis and their columns may appear in the Intact Deconvolution Results table.
Parameters specific to the "Automatic Peak Detection" and "Sliding Windows" deconvolution methods for the Xtract and ReSpect algorithms for intact mass analysis experiment are not applicable to top down analysis.
Each peak-specific Intact Deconvolution Results table is organized by components at the upper level, followed by raw data files (for multiconsensus experiments) at the next level, and then by charge states at the lowest level. You must select a peak from the Peak Selection list in the table title bar to display the results specific to the selected peak.
NOTE
Because the BioPharma Finder application uses an updated algorithm, the calculated mass values in the Results table from this application might be slightly different from those reported by the Protein Deconvolution application.