The following table describes the parameters of the Variable Modifications for Intact and Peptide Analysis pane in the Protein Sequence Editor.
Parameter | Description |
---|---|
Max # Modifications | Displays the maximum number of variable modifications that you want to assign to the target protein sequence. |
Intact Protein | Displays the maximum number of variable modifications for Intact Mass Analysis. |
Peptide Mapping | Displays the maximum number of variable modifications for Peptide Mapping Analysis. |
Glycosylation | Specifies the type of glycosylation assigned to the target protein sequence. |
N, O Glycan | Lists the types of glycosylations that you can apply to the N-linked and O-linked glycans. The application supports O-glycans only for Peptide Mapping Analysis. |
Modifications | Lists the modifications available to apply to an N terminus, C terminus, or side chain. |
N Terminal | Displays information about the N-terminal modification that you selected from the list. |
Fields | |
Mono. Mass | Displays the monoisotopic mass of the selected N-terminal modification. |
Avg. Mass | Displays the average mass of the selected N-terminal modification. |
Buttons | |
Add | Adds the selected modification to the Modifications Selected for Search list. |
Remove | Removes the selected modification from the Modifications Selected for Search list. |
Load Default Mods | Quickly loads the sublist of default modifications into the Modifications Selected for Search list. |
C Terminal | Displays information about the C-terminal modification that you selected from the list. |
Fields | |
Mono. Mass | Displays the monoisotopic mass of the selected C-terminal modification. |
Avg. Mass | Displays the average mass of the selected C-terminal modification. |
Buttons | |
Add | Adds the selected modification to the Modifications Selected for Search list. |
Remove | Removes the selected modification from the Modifications Selected for Search list. |
Load Default Mods | Quickly loads the sublist of default modifications into the Modifications Selected for Search list. |
Side Chain | Displays information about the side chain modification that you selected from the list. |
Fields | |
Mono. Mass | Displays the monoisotopic mass of the selected side chain modification. |
Avg. Mass | Displays the average mass of the selected side chain modification. |
Residues | Displays the amino acid residue for the selected modification. |
Buttons | |
Add | Adds the selected modification to the Modifications Selected for Search list. |
Remove | Removes the selected modification from the Modifications Selected for Search list. |
Load Default Mods | Quickly loads the sublist of default modifications into the Modifications Selected for Search list. |