For peptide mapping analysis and oligonucleotide analysis, the following occurs after you save the processing method:
- If you previously entered the experiment name and uploaded one or more raw data files for the experiment on the Peptide Mapping Analysis page or the Oligonucleotide Analysis page. The application saves the method, opens the Queue page, and places your job in the run queue.
- If the queue is not paused, and the queue is empty or all queued jobs are already completed, the application immediately processes the experiment using the saved method information. Otherwise, your job waits in the run queue until you resume the queue processing or your job moves to the top of the queue.
- If you did not previously specify the experiment name and raw data files, the application saves the method and transfers you back to the Peptide Mapping Analysis page or the Oligonucleotide Analysis page. This page displays the saved method in the Processing Method area, and you can select this method to use for processing a new experiment.
For intact mass analysis, the application saves the method and transfers you back to the Intact Mass Analysis page. This page displays the saved method in the Processing Method area, and you can select this method to use for processing a new experiment.
For top down analysis, the application saves the experiment parameters to the database. If you use the same name as an existing experiment, it overwrites that experiment. It also saves the method parameters to a custom method, except for the protein sequence information, and then transfers you to the Queue page to start processing the experiment. The next time you load the Top Down Analysis page, it displays the saved method in the Processing Method area, and you can select this method to use for processing a new experiment. Before processing, you must reselect a protein sequence for each peak in the method from the list of sequences that you select on the Top Down Analysis page.
The application saves all methods that you create to a database.