Prerequisites

  1. To display multiple chromatogram plot types for the same raw data file in the Chromatogram pane:

Procedure

  1. Open the results of your experiment from the Queue page or from the Load Results page.
  2. The Process and Review page opens by default and displays the results of your oligonucleotide analysis experiment, including the chromatograms.
  3. Right-click the Chromatogram pane and select Select Chromatogram.
  4. The Select Chromatogram dialog box opens.
  5. Your raw data file selections in the Select Chromatogram dialog box control the chromatogram plots. To return the control of the chromatogram plots to your selections in the Results table, you must clear all raw data file selections in this dialog box and then select OK. Otherwise, proceed.
  6. If you select only one plot type from the list on the left, you can select multiple raw data files from the list on the right.
  7. Conversely, to select multiple plot types, you must select only one raw data file.
  8. image/svg+xml
    Select Chromatogram dialog box
  9. Select the appropriate checkboxes from the list on the left side of the dialog box.
  10. The available chromatogram plot types include:
  11. •Selected Ion Chromatogram (SIC)
  12. •Total Ion Chromatogram (TIC)
  13. •Base Peak Chromatogram (BPC)
  14. •Analog
  15. •r-TIC (TIC after the removal of background ions)
  16. •r-BPC (BPC after the removal of background ions)
  17. On the right side of the dialog box, select a single raw data file from the list.
  18. image/svg+xml
    Selecting multiple chromatogram plots for a single raw data file
  19. Select OK to update the chromatogram plots.
  20. The Chromatogram pane displays the selected plot types stacked on top of each other for the selected raw data file.
Different types of chromatograms displayed for a single raw data file
image/svg+xml