Prerequisites
- For an oligonucleotide analysis experiment, you entered an experiment name, loaded the raw data files and entered the conditions (if applicable), and selected an oligonucleotide sequence on the Oligonucleotide Analysis page.
Procedure
- In the Processing Method area of the Oligonucleotide Analysis page, select a processing method in the table to edit and select Edit Method.
- The processing method editing wizard opens the Component Detection page under the Parameters tab, showing the component detection parameters in the open method.
- To create a custom method, modify the parameters in a default method and save your specifications using a different name.
- If your experiment contains multiple raw data files, the BioPharma Finder application determines the component detection and identification parameters for the whole group.
- Use the processing method editing wizard on the Component Detection, Identification, and Customize Results pages to specify the appropriate parameters for the processing method.
- See the following topics:
- •Edit the Component Detection page
- •Edit the Identification page
- •Edit the Customize Results page
- •Edit the Save Method page
- The application automatically determines several parameters for the experiment, such as the absolute signal threshold and retention time shift, using data in the raw data files.
- When you are finished editing the parameters on each page, select Next in the command bar to advance to the next page.
- On the last page, the Save Method page, save the modified processing method.
- See Save a processing method.
NOTE
If you edit a default processing method, you must save your customized method under a new name.
You cannot overwrite a default processing method.
- The application saves the processing method and the method appears in the list in the Processing Method area on the Oligonucleotide Analysis page. You can then select the method to process your current and future experiments.