The first page of the processing method editing wizard is the Component Detection page. Use the Component Detection page to change the parameters related to component peak detection in your analysis.

Prerequisites

  1. For an oligonucleotide analysis experiment, you entered an experiment name, loaded the raw data files and entered the conditions (if applicable), and selected an oligonucleotide sequence on the Oligonucleotide Analysis page.

Procedure

  1. To open the Component Detection page, select a processing method in the Processing Method area on the Oligonucleotide Analysis page by selecting the checkbox.
  2. Select Edit Method to use the processing method editing wizard.
  3. The processing method editing wizard opens on the Parameters page and displays the first subpage, Component Detection. The Parameters page contains four subpages (Component Detection, Identification, Customize Results, and Save Method) that can be accessed by selecting the appropriate subtab.
  4. The Component Detection page contains four sections containing editable parameters: Select Task to be Performed, Peak Detection, Ion Alignment, and Mass Measurement.
  5. Select the Show Advanced Parameters checkbox at the bottom left side of the Component Detection page to display additional parameters you can edit (if needed).
  6. Enter the appropriate parameter values.
  7. For more information on these parameters, see Component Detection page layout and parameters.
  8. On the right side of the Component Detection page, you can visualize the read-only Absolute MS Signal Threshold parameter in the base peak chromatogram. See View and edit the signal threshold.
  9. The Select Task to be Performed drop-down menu lists the tasks that the application can perform. Depending on the task selected, the results on the Process and Review page will be displayed in two tables (Consolidated Results and Charge State Results for the Find All Masses in the Run task) or in a single Charge State Results table (for all other task types).
  10. When you are finished editing the parameters on the Component Detection page, select Next on the command bar to advance to the Identification page.