The following table describes the types of information available on the Modification Summary page.
Parameter | Description |
|---|---|
Modification Results table | Displays the recovery status and abundance of all detected modifications to oligonucleotides in the analysis. |
Components table | Displays component-level information for a selected modification. |
Modification plot | Displays the modification abundance percentages for the modifications selected in the Modification Results table, grouped by conditions/raw data file names. |
% Abundance (y axis) | Displays the percentages of abundance for the selected modifications in the Modification Results table. |
Condition−Raw data file (x axis) | Displays the names of all conditions and their corresponding raw data files loaded for the experiment. |
Process and Review panes (tabbed vertically on the right side of the Modification Summary page) | |
Chromatogram pane | Displays the chromatograms for the component or raw data file selected in the Modification Results table. This chromatogram is similar to the chromatogram on the Process and Review page. |
Relative Intensity (y axis) | Displays the ratio of the intensity of a specific peak to the intensity of the peak with the highest intensity. |
RT (min) (x axis) | Displays the retention time (RT) of the spectrum, which is the time after injection at which a compound elutes. Retention time can also refer to the total time that the compound is retained on the chromatographic column. |
Oligo Match ID Details pane | Provides detailed fragment information for a selected component when MS2 data is available. Editable in real-time. This information is similar to the Oligo Match ID Details on the Process and Review page. |
Trend Ratio pane | Displays the bar plots for the ratios between various conditions and the reference condition for the component selected in the Modification Results table. These plots are similar to the trend ratio plots on the Process and Review page. |
Ratio value (y axis) | Displays the Avg MS Area value for a particular condition/Avg MS Area value for the reference condition. |
Ratio conditions (x axis) | Displays the names of the various conditions and the name of the reference condition. |
Trend MS Area pane | Displays the bar plots for the MS Area values for the component selected in the Modification Results table for each raw data file used in the experiment. These plots are similar to the trend MS area plots on the Process and Review page. |
MS Area value (y axis) | Displays the MS Area values from each of the individual raw data files assigned to a particular condition. |
Condition−Raw data file (x axis) | Displays the names of the various conditions and their assigned raw data files used in the experiment. |
Oligo Sequence Coverage pane | Displays the Fragment Coverage Map, including the oligonucleotide sequence information and the color-coded fragment ions. This map is similar to the fragment coverage map on the Process and Review page. |
Oligo Sequence pane | Displays the oligonucleotide sequence assigned to the experiment, highlighting the nucleotide sequence corresponding to the component selected in the Modification Results table. This sequence is similar to the oligonucleotide sequence on the Process and Review page. |
Full Scan Spectra pane | Displays the deconvoluted and full-scan spectra with mass and m/z information. These spectra are similar to the deconvoluted and full scan spectra on the Process and Review page. |
Relative Abundance (y axis) | Displays the ratio of the abundance of a specific peak to the abundance of the peak with the highest abundance. |
Mass or m/z (x axis) | Displays the mass or mass-to-charge ratio of the ions formed from molecules. |
MS2 Spectra pane | Displays the predicted spectrum stacked on top of the experimental spectrum. This spectrum is similar to the MS2 spectrum on the Process and Review page. |
Relative Abundance (y axis) | Displays the ratio of the abundance of a specific peak to the abundance of the peak with the highest abundance. |
m/z (x axis) | Displays the mass-to-charge ratio of ions formed from molecules. |