The Full Scan Spectra pane displays the noise-reduced deconvoluted and full-scan spectrum for each component in your analysis.

Procedure

  1. Open the results of your experiment from the Queue page or from the Load Results page.
  2. See Open results.
  3. The Process and Review page opens by default and displays the results of your oligonucleotide analysis experiment, including the deconvoluted and full-scan spectra..
  4. On the left side of the Process and Review page, select the Full Scan Spectra tab.
  5. Deconvoluted and full-scan spectra in the Full Scan Spectra pane
    Deconvoluted and full-scan spectra in the Full Scan Spectra pane
  6. The Full Scan Spectra pane displays the noise-reduced deconvoluted spectrum stacked above the full-scan spectrum.
  7. In the deconvoluted spectral plot, the x axis represents the mass. Above the plot, the exact retention time (RT) value is listed at the end of the file information.
  8. In the full-scan spectral plot, the x axis represents the mass-to-charge ratio (m/z). The full-scan plot also shows the charge states of the identified ions, labeled in red. Above the plot, the signal-to-noise ratio (S/N) is listed at the end of the file information.
  9. In both plots, the y axis indicates the relative abundance.
  10. Both plots label the centroid peaks with their individual mass or m/z values, with four decimal digits for high-resolution data and two decimal digits for low-resolution data.
  11. For more information on the commands available in the Full Scan Spectra pane, see Full Scan Spectra pane commands.
  12. Do one of the following:
  13. Select the row of a component in the Results table.
  14. The available spectral plots in the Full Scan Spectra pane display data from the reference raw data file.
  15. By default, the application considers the first raw data file in an experiment as the reference file.
  16. For an experiment with multiple raw data files, expand (+/− icon) a component row in the Results table and select the row for a single raw data file.
  17. The available spectral plots in the Full Scan Spectra pane display data from the selected raw data file.
  18. Select a scan (specific retention time (RT) point) on the base peak chromatogram (BPC) in the Chromatogram pane.
  19. The available spectral plots in the Full Scan Spectra pane display data from the selected scan.