On the Process and Review page, the Oligo Match ID section provides detailed fragment information for a selected component when MS2 data is available.

Procedure

  1. Open the results of your experiment from the Queue page or from the Load Results page.
  2. See Open results.
  3. The Process and Review page opens by default and displays the results of your oligonucleotide analysis experiment, including the oligo match ID results.
  4. On the top left side of the Process and Review page, select the Oligo Match ID Details tab (tabbed together with the Chromatogram pane).
  5. Oligo Match ID pane
    Oligo Match ID pane
  6. The Oligo Match ID Details pane opens and contains two expandable sections: Select Fragments and Component Summary.
  7. The Select Fragments section contains the following parameters: Ion Series, Internal Fragments, and Charge States.
  8. Select a component row in the Results table to edit the Select Fragments and Component Summary sections of the Oligo Match ID Details tab.
  9. In the Select Fragments section, select the checkbox for the fragments you want to display in the Component Summary table below and select the Apply button.
  10. The Component Summary table updates according to the parameters specified in the Select Fragments section.
  11. On the top right side of the page, select the MS2 Spectra tab.
  12. MS2 spectrum containing fragment information for a selected component
    MS2 spectrum containing fragment information for a selected component
  13. The MS2 spectrum appears for the component selected in the Results table, with the selected fragment types labeled.
  14. At the top of the MS2 Spectra pane, the Activation list provides the available fragmentation types and the Resolution list provides the available resolution types for the MS2 spectrum.
  15. Edit the Select Fragments section as necessary to view the Component Summary results and fragment-labeled MS2 spectra for the selected component.