Use the Random Sequence Generator in the Oligonucleotide Sequence Editor to create random sequence variations of an oligonucleotide sequence. These random sequences are analyzed along with the original input oligonucleotide sequence so that you can confidently identify and analyze the components displayed on the Process and Review page after experiment processing.

Procedure

  1. To open the Oligonucleotide Sequence Editor window, from the BioPharma Finder home page, double-click Sequence Manager in the Experiment Types area.
  2. In the Sequence Manager, select an oligonucleotide sequence by double-clicking the sequence name.
  3. The Oligonucleotide Sequence Editor opens in a new window.
  4. For more information about the panes and parameters in this window, see Oligonucleotide Sequence Editor parameters.
  5. In the Sequence Information pane of the Oligonucleotide Sequence Editor window, type the number of randomized sequences you want to include in your analysis in the Random Sequence Generator box.
  6. You can include up to five random sequences.
  7. Select Apply.
  8. The Oligo Sequence Map pane displays the original oligonucleotide sequence along with any randomized sequences.
  9. Each randomized sequence is named using the name of the original oligonucleotide with “Randomized” and its sequence number appended to the end.
  10. For example, for an oligonucleotide sequence “15mer”, the first randomized sequence would be named “15mer_Randomized_1”, as shown in the figure below.
  11. Random Sequence Generator in the Oligonucleotide Sequence Editor
    Random Sequence Generator in the Oligonucleotide Sequence Editor
  12. Select Save or Save as New in the command bar to save the modifications. Or, if you do not want to save the modifications to the sequence, select Cancel.
  13. See Save an oligonucleotide sequence.
  14. The original oligonucleotide sequence is saved with the added randomized sequences in the same file for processing.