The first page of the processing method editing wizard is the Component Detection page. Use the Component Detection page to change the parameters related to component peak detection in your analysis.

Prerequisites

  1. For an intact mass analysis experiment, you entered an experiment name, loaded the raw data files and entered the conditions (optional), and selected a protein or oligonucleotide sequence (optional) on the Intact Mass Analysis page.

Procedure

  1. To open the Component Detection page, select a processing method in the Processing Method area on the Intact Mass Analysis page by selecting the checkbox.
  2. Select Edit Method to use the processing method editing wizard.
  3. The processing method editing wizard opens on the Parameters page and displays the first subpage, Component Detection. The Parameters page contains four subpages (Component Detection, Identification, Report and Save Experiment) that can be accessed by selecting the appropriate subtab.
  4. On the Component Detection page, the left side of the page displays the editable parameters related to component detection in your intact mass analysis experiment. These parameters vary, depending on the deconvolution algorithm (Xtract or ReSpect) and source spectra (deconvolution) method (average over selected retention time, automatic peak detection, or sliding windows) that you select. See Specify parameters on the Component Detection page.
  5. On the right side of the page, the chromatogram and source spectrum for each raw data file in the experiment are displayed. See Plots on the Component Detection page.
  6. Select the Show Advanced Parameters checkbox at the bottom left side of the Component Detection page to display additional parameters you can edit (if needed) and enter the appropriate parameter values.
  7. When you are finished editing the parameters on the Component Detection page, select Next on the command bar to advance to the Identification page.