The Results table on the Process and Review page displays information about the components identified in your intact mass analysis experiment, including identifications, modifications, sequence information, retention times, and more. Selecting a component row in a Results table populates the other panes on the Process and Review page with information related to the selected component.

Procedure

  1. Open the results of your experiment from the Queue page or from the Load Results page.
  2. See Open results
  3. The Process and Review page opens by default and displays the results of your intact mass analysis experiment in the Results table.
  4. By default, the table displays results at the component level (top-level data).

  5. NOTE

    The columns and levels in the Results table varies depending on the selected results format (Batch or Multiconsensus), deconvolution algorithm (ReSpect or Xtract), deconvolution method (average over selected retention time, automatic peak detection, or sliding windows) and other processing method parameters.

    Batch experiments (or experiments with a single raw data file) contain two levels of results: Component and Charge State.

    Multiconsensus experiments contain three levels of results: Component, Raw Data File, and Charge State.

    However, batch experiments (or experiments with a single raw data file) with drug-to-antibody ratio (DAR) feature enabled, also have three levels (Component, Raw Data File, and Charge State) and the Results table is similar to the Results table for a multiconsensus experiment.

  6. image/svg+xml Componentlevel Charge Statelevel
    Results table for a single raw data file experiment (two levels)
  7. See Results table parameters (for single file or batch format experiments).
  8. image/svg+xml Componentlevel Charge Statelevel Raw data filelevel
    Results table for a multiconsensus experiment (three levels)
  9. See Results table parameters (multiconsensus format experiments).
  10. Select a row in the table to populate the other panes in on the Process and Review page with information related to the selected component.
  11. (Optional) Select the plus icon (+) in the left margin of a component row to display the lower levels (raw data file- or charge state-level) of information for the selected component.
  12. image/svg+xml More Identification matiches inthe Matched Sequence Pane Protein sequence and modification informationfor the best identification match
    Results for a single-raw data file/batch experiment with target sequence matching
  13. (Optional) For a drug-antibody ratio (DAR)-enabled experiment, select the plus icon (+) in the left margin of a component row to display the raw data file-level Drug Load results related to the top-level component.
  14. When you change the Drug Load value in the Matched Sequence pane, the value automatically updates in the Results table.
  15. See View the matched sequence information.
Results table showing the results of a DAR-enabled experiment
image/svg+xml Drug load values Calculated DAR values inthe Average DAR pane
  1. You can change the reference mass used in the Results table as necessary.
  2. See Change the reference mass.
  3. You can export the Results table as a Microsoft™ Excel™ workbook (.xlsx) or Json file. You can also save selected components to an intact workbook for use in the Chromeleon™ chromatography data system.
  4. See Export the Results table and Save an intact workbook.

TIP

On the Process and Review page, you can use real-time optimization to quickly change processing method parameters and update the data in the Results table.

In the intact mass analysis workflow, you cannot use RTO to reprocess results for an auto peak detection experiment.

You can only use the RTO feature for average over selected retention time and sliding windows experiments.