Create or edit a processing method - BioPharma Finder Software - Create or edit a processing method - BioPharma Finder Software - BioPharma Finder Software

Prerequisites

1. For an intact mass analysis experiment, you entered an experiment name, loaded the raw data files and entered the conditions (optional), and selected a protein or oligonucleotide sequence (optional) on the Intact Mass Analysis page.

Procedure

1. In the Processing Method area of the Intact Analysis page, select a processing method in the table and select Edit Method.
2. The processing method editing wizard opens the Component Detection page under the Parameters tab, showing the component detection parameters in the open method.
2. To create a custom method, modify the parameters in a default method and save your specifications using a different name.
3. Use the processing method editing wizard on the Component Detection, Identification, and Report pages to specify the appropriate parameters for the processing method.
4. See the following topics:
5. •Edit the Component Detection page
6. •Edit the Identification page
7. •Edit the Report page
8. •Edit the Save Method page
9. For a batch experiment, the application derives the m/z range parameter value based on information retrieved from each individual raw data file. For a multiconsensus experiment with multiple raw data files, by default, the application uses the above information from the first loaded raw data file.
10. The application automatically determines the other component detection and identification parameters for the experiment and displays these parameters on the pages under the Parameters tab. You can edit these parameters as needed.
11. The parameters in the processing method editing wizard vary, depending on the deconvolution algorithm (Xtract or ReSpect) and source spectra (deconvolution) method (average over selected retention time, automatic peak detection, or sliding windows) that you select.
4. When you are finished editing the parameters on each page, select Next in the command bar to advance to the next page.
5. On the last page, the Save Method page, save the modified processing method.
6. See Save a processing method.

7. NOTE

   If you edit a default processing method, you must save your customized method under a new name.

   You cannot overwrite a default processing method.

8. The application saves the processing method and the method appears in the list in the Processing Method area on the Intact Mass Analysis page. You can then select the method to process your current and future experiments.