Use the intact mass analysis workflow in the BioPharma Finder application to determine the molecular mass of an intact protein or oligonucleotide.
Electrospray ionization (ESI) of intact proteins, peptides, and oligonucleotides produces mass spectra that contain series of multiply charged ions with associated mass-to-charge ratio (m/z) values. The resulting spectrum is complex and difficult to interpret, requiring mathematical algorithms for the analysis of the data.
Through a process called deconvolution, the BioPharma Finder application uses such algorithms to transform a charge state series into a molecular mass for an intact protein or oligonucleotide. To do this, the application identifies multiple peaks in the mass spectrum associated with different charge states of the same component and returns information about the masses and abundance of that component.