The following table describes the parameters in the Assign Variable Modifications pane in the Oligonucleotide Sequence Editor.

Parameters descriptions for the Assign Variable Modifications pane

Parameter

Description

Max # Modifications

Specifies the maximum number of variable modifications that you want to assign to the target oligonucleotide sequence.

Range: 0 to 8; default: 1

Modifications

Lists the modifications available to apply to the 5'-terminal, 3'-terminal, and oligonucleotide building blocks.

5'-Terminal

Displays information about the 5'-terminal modification that you selected from the list.

Read-only fields

Mono. Mass

Displays the monoisotopic mass of the selected 5'-terminal modification to three decimal places.

Avg. Mass

Displays the average mass of the selected 5'-terminal modification to two decimal places.

Buttons

Add

Adds the selected modification to the Modifications Selected for Search list.

Remove

Removes the selected modification from the Modifications Selected for Search list.

Load Default Mods

Quickly loads the sublist of default modifications into the Modifications Selected for Search list.

3'-Terminal

Displays information about the 3'-terminal modification that you selected from the list.

Read-only fields

Mono. Mass

Displays the monoisotopic mass of the selected 3'-terminal modification to three decimal places.

Avg. Mass

Displays the average mass of the selected 3'-terminal modification to two decimal places.

Buttons

Add

Adds the selected modification to the Modifications Selected for Search list.

Remove

Removes the selected modification from the Modifications Selected for Search list.

Load Default Mods

Quickly loads the sublist of default modifications into the Modifications Selected for Search list.

Oligo Variable Modifications

Displays information about the oligo variable modification that you selected from the list.

Read-only fields

Mono. Mass

Displays the monoisotopic mass of the selected oligo variable modification to three decimal places.

Avg. Mass

Displays the average mass of the selected oligo variable modification to two decimal places.

Residues

Displays residue for the selected oligo variable modification.

Buttons

Add

Adds the selected modification to the Modifications Selected for Search list.

Remove

Removes the selected modification from the Modifications Selected for Search list.

Load Default Mods

Quickly loads the sublist of default modifications into the Modifications Selected for Search list.