Mass-only sequences are useful when you want the application to identify detected components (such as known impurities) with a known mass or formula in an intact mass analysis experiment.
Procedure
- Open the Sequence Manager by selecting Sequence Manager on the BioPharma Finder home page.
- Select New Oligonucleotide to the right of the sequence table.
- The Oligonucleotide Sequence Editor opens with the Manual Input Sequence pane expanded.
- In the Name box, type the name of the oligonucleotide sequence.
- In the Category drop-down list, select Intact Deconvolution.
Sequence Information pane input for a formula-only oligonucleotide sequence - Do one of the following:
- To create a formula-only sequence, enter the chemical formula of the sequence in the Formula box, and then select Apply.
- The application automatically calculates the monoisotopic mass and average mass of the sequence from the user-specified formula.
- While entering the formula, the limit for both Monoisotopic Mass and Average Mass is 999999999 for each element.
- If you enter anything larger than 999999999 for each element, the application displays an error message that it is an unsupported element
- To create a mass-only sequence, enter the monoisotopic mass and the average mass of the sequence in the respective boxes.
- The Apply button is not available for a mass-only sequence.
- Save the sequence.
- See Save an oligonucleotide sequence.
- The application adds the saved sequence to the table on the Sequence Manager page and appears as a sequence option of the Intact Mass Analysis page.
NOTE
You cannot assign variable modifications to a mass-only or formula-only sequence.
