Within the default list of modifications, the BioPharma Finder application sets the modifications shown in the following table as a default sublist that you can quickly load to assign 5'-terminal, 3'-terminal, backbone linker, and 2'-ribose sugar group modifications to an oligonucleotide sequence.
5’-terminal modifications: |
Dephosphorylation |
3’-terminal modifications: |
Phosphorylation |
Base modifications: |
Adenine loss (A) |
Uracil loss (U) |
Cytosine loss (C) |
Guanine loss (G) |
Thymine loss (T) |
Deamination (AC) |
Oxidation (ACGU) |
G Depurination (G) |
U Depyrimidination (U) |
C Depyrimidination (C) |
T Depyrimidination (T) |
I Depurination (I) |
Acetyl group (AGC) |
Benzoyl group (AC) |
Isobutyl group (G) |
Backbone linker modifications: |
Cnet (ps) |
Sulfur loss (s) |
Phosphorothioate conversion (s) |
2’-ribose modifications: |
HF loss |