Use ICIS peak detection - Ardia Data Viewer - Use ICIS peak detection - Ardia Data Viewer

Apply To All Plots checkbox

Applies the current chromatogram peak identification and integration settings to all displayed plots.

To apply the criteria to only the active plot, clear this option.

Reset button

Restores the default settings.

Apply button

Applies the chromatogram peak identification and integration settings to the selected peak.

Peak parameters

Baseline Window

Specifies the number of scans the peak detection algorithm uses for determining the minimum baseline for the peak. A higher number of scans means a wider scan range, resulting in a lower baseline.

Range: 1–500; default: 40

Area Noise Factor

Specifies the noise level multiplier that the peak detection algorithm uses to determine the peak edge after the location of the possible peak.

Range: 1–500; default: 5

Peak Noise Factor

Specifies the noise level multiplier that the peak detection algorithm uses to determine the signal threshold of a potential peak.

Range: 1–1000; default: 10

Constrain Peak Width checkbox

Limits the peak width of a component during the peak integration of a chromatogram. You can then set values that control when peak integration turns on and off by specifying a peak height threshold and a tailing factor.

Peak Height (%)

Specifies how much above the baseline (as a percentage) the total peak height of a signal must be before integration turns on or turns off.

Range: 0.00–100.0; default: 5

Tailing Factor

Specifies a factor that controls how the algorithm integrates the tail of a peak. This factor is the maximum ratio of the trailing edge to the leading edge of a constrained peak.

Range: 0.50–9.0; default: 1

Advanced

Manual Noise Region

Specifies a region of the chromatogram that the peak detection algorithm uses to determine noise. See Select the manual noise region.

RT Range

Specifies the RT range that the algorithm uses to determine noise. The RT range must be within the chromatogram range.

Noise Method

ICIS peak detection uses one of these two algorithms for signal-to-noise ratio (S/N) estimation:

• INCOS algorithm: A single-pass algorithm that determines the noise level of a chromatogram. For more information, see INCOS algorithm.
• Repetitive algorithm: A multiple-pass algorithm that determines the noise level of a chromatogram. In general, this algorithm is more accurate in analyzing the noise than the INCOS algorithm; however, it might take longer depending on the data. For more information, see Repetitive algorithm.

RMS

Calculates noise as a root mean square (RMS). The algorithm uses the peak-to-peak method for the noise calculation.

When you determine the noise region manually, the algorithm automatically selects RMS.

Min Peak Width

Specifies the minimum number of scans required in a peak.

Range: 0–500; default: 3

Multiplet Resolution

Specifies the minimum separation in scans between the apexes of two potential peaks. This criterion determines if two peaks are resolved.

Range: 1–500; default: 10

Area Tail Extension

Specifies the number of scans past the peak endpoint that the application uses in averaging the intensity.

Range: 0–100; default: 5

Area Scan Window

Specifies the number of scans on each side of the apex that the application includes in the integration area.

Range: 0–100; default: 0

NOTE Zero scans means that the algorithm includes all scans in the integration area, from the start to the end of the peak.

Pharmacopoeia S/N

Pharmacopoeia S/N calculation

Specifies a calculation that overwrite the signal to noise calculation from ICIS peak detection settings. For more information, see Appendix: Pharmocopoeia S/N.

Time span multiply factor

Specifies the factor (n) for multiplying the peak width at half height to calculate the time interval. For more information, see Multiple of time span method.