The following table defines advanced settings used in the ICIS algorithm for peak detection.

Parameter

Subparameter

Description

Manual Noise Region

Checkbox

Specifies a region of the chromatogram that the peak detection algorithm uses to determine noise.

RT Range

N/A

Specifies the RT range that the algorithm uses to determine noise. The RT range must be within the chromatogram range.

Noise Method

N/A

Specifies the algorithms for S/N estimation.

INCOS

Radio button

A single-pass algorithm that determines the noise level of a chromatogram.

Repetitive

Radio button

A multiple-pass algorithm that determines the noise level of a chromatogram. In general, this algorithm is more accurate in analyzing the noise than the INCOS algorithm; however, it might take longer depending on the data.

RMS

Checkbox

Calculates noise as a root mean square (RMS). The algorithm uses the peak-to-peak method for the noise calculation.

When the Manual Noise Region option is selected, the algorithm automatically selects the RMS option.

Min Peak Width

N/A

Specifies the minimum number of scans required in a peak.

Range: 0–500; default: 3.

Multiplet Resolution

N/A

Specifies the minimum separation in scans between the apexes of two potential peaks. This criterion determines if two peaks are resolved.

Range: 1–500; default: 10.

Area Tail Extension

N/A

Specifies the number of scans past the peak endpoint the application uses to average the intensity.

Range: 0–100; default: 5.

Area Scan Window

N/A

Specifies the number of scans on each side of the apex that the application includes in the integration area.

Range: 0–100; default: 0.