Check the vial positions for the needle wash station, the input and output well plates, as well as the different solutions trays to ensure that the tool can accurately access the vial positions and teach points for the different modules.

NOTE

If no reagents are available, instead of checking vial positions, proceed with loading the IQ kit and 3 point check teaching of tray B1 and tray A teaching (see Check tray A teaching and see Check tray B1 teaching). The vials will have to be relocated during the check teaching procedure for tray B1.

Ensure to load the following components in the correct positions:

  • Input well plate on top of the thermal mixer
  • Output well plate in tray A slot
  • Reagents tray in tray B1 slot
  • TMT or TMT pro tray in tray B2 slot
  • D buffers tray in tray D slot
  • SPE cartridge tray in μSPE slot

NOTE

To check the vial positions on trays containing solution vials, ensure to have vials present in the left back, left front, and right front edge positions of each tray. Three vials are required for each module.

Perform the vial positions check in the User level.

For the PAL terminal button functions, refer to the PAL terminal section.

Steps

  1. On the PAL terminal screen, select Options and select Maintenance and select Check Vial Positions.
    2. Select maintenance
    Select maintenance
    Select check vial positions
    Select check vial positions
  2. Select Check.
    3. Select check
    Select check
  3. The tool checks the vial positions on the following trays in succession, automatically:
  • Input well plate on thermal mixer (check position along x and y axis)
    • Check vial positions on input well plate
    Check vial positions on input well plate
    Thermal mixer first vial position
    Thermal mixer first vial position
    Thermal mixer second vial position
    Thermal mixer second vial position
    Thermal mixer third vial position
    Thermal mixer third vial position
  • SPE cartridge tray (check positions along x, y, and z axis)
    • Check vial positions on tray SPE
    Check vial positions on tray SPE
    SPE tray first vial position
    SPE tray first vial position
    SPE tray second vial position
    SPE tray second vial position
    SPE tray third vial position
    SPE tray third vial position
  • D buffers tray (check positions along x, y, and z axis)
    • Check vial positions on tray D buffers
    Check vial positions on tray D buffers
    Tray D buffers first vial position
    Tray D buffers first vial position
    Tray D buffers second vial position
    Tray D buffers second vial position
    Tray D buffers third vial position
    Tray D buffers third vial position
  • Needle wash station (check positions along x, y, and z axis)
    • Check vial positions on needle wash station
    Check vial positions on needle wash station
    Needle wash station first vial position
    Needle wash station first vial position
    Needle wash station second vial position
    Needle wash station second vial position
  • TMT tray in tray B2 slot (check positions along x, y, and z axis)
    • Check vial positions on tray B2
    Check vial positions on tray B2
    Tray B2 first vial position
    Tray B2 first vial position
    Tray B2 second vial position
    Tray B2 second vial position
    Tray B2 third vial position
    Tray B2 third vial position
  • Reagents tray in tray B1 slot (check positions along x, y, and z axis)
    • Check vial positions on tray B1
    Check vial positions on tray B1
    Tray B1 first vial position
    Tray B1 first vial position
    Tray B1 second vial position
    Tray B1 second vial position
    Tray B1 third vial position
    Tray B1 third vial position
  • Output well plate in tray A slot (check positions along x and y axis)
Check vial positions on output well plate
Check vial positions on output well plate
Tray A first vial position
Tray A first vial position
Tray A second vial position
Tray A second vial position
Tray A third vial position
Tray A third vial position
  • Press OK. This moves the robot arm back to the home position.

    • NOTE

    If the tool can not accurately access any vial position on any tray, check the teaching for that module (see Optional check teaching).